2-Thio Amidites and Supports

Select a Product:

2-Thio deoxy Uridine 3'-lcaa CPG 1000Å
2-Thio deoxy Uridine 3'-lcaa CPG 500Å
2-Thio deoxy Uridine CED phosphoramidite
2-Thio Thymidine 3'-lcaa CPG 1000Å
2-Thio Thymidine 3'-lcaa CPG 500Å
2-Thio Thymidine CED phosphoramidite

Application Notes

Thiolated Nucleobases:

  • Thiolated nucleobases has been successfully used in template-directed covalent cross-linking of DNA. This strategy is based on the synthetic incorporation of thionucleic acids into oligodeoxynucleotides. In subsequent, post-synthetic modifications of the non-natural bases, reactive functionality is tethered in a site specific, chemoselective manner via the thiocarbonyl group-by S-alkylation or disulfide formation-without affecting other bases within the DNA strand. This strategy makes possible the divergent incorporation of reactive functionality that would otherwise be incompatible with solid-phase synthesis conditions.1
  • Another high demand for thiolated nucleosides is in DNA and RNA structural study due to size and electro negativity difference of the sulfur atom compared to oxygen.2

References:

1. Coleman, R. S., Kesicki, E. A. Jour. Am. Chem. Soc. 1994, 116, 11636.

2. Sismour, A. M., Benner, S. A. Nucleic. Acids Res. 2005, 33, 5640.

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