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Application Notes
Iso-Cytidine (IsoC) and Iso-Guanosine (IsoG) Phosphoramidites and Supports:
Iso-Cytidine and Iso-Guanosine are variants of natural Cytidine and Guanosine bases. One of the key advantages of these monomers is their capability of binding with each other with three hydrogen bonds as shown in the Figure 1. Additionally these monomers do not recognize naturally occurring Cytidine and Guanosine bases. Thus they are used for specific sequence based genetic engineering.1,2
Figure 1: Schematic illustration of Iso-C and Iso-G base pairing.
References:
1. Switzer, C. Y.; Moroney, S. E.; Benner, S. A. Biochemistry 1993, 32, 10489-10496 & references cited in this article.
2. Chen, X.; Kierzek, R.; Turner, D. H. J. Am. Chem. Soc. 2001, 123, 1267-1774.