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Product
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Avg. MW of a single base unit ~ 330 Daltons:

To determine the Avg. MW of an oligonucleotide:
  • MW= [(#dA x313.21) + (#dC x289.19) + (#dG x329.21) + (#T x304.20) - 61.96]

    # of nanomoles in an oligonucleotide:
  • (A260nm x 90) / (# of bases)

    Useful Oligo Conversion:
  • 1 A260 unit = 33 ug/ml of ssDNA
  • 1 A260 unit = 50 ug/ml of dsDNA
  • 1 A260 unit = 40 ug/ml of RNA

    Sample Quantitation of Synthetic Oligomers:
  • 20-mer DNA: 1 A260nm = 33 ug / (20 x325g/mol) x 10-6 = 5nmol
  • 40-mer DNA: 1 A260nm = 33 ug / (40 x325g/mol) x 10-6 = 2.5nmol

    Useful Primer Conversions:
  • from pmol to ug: ug = (# of pmol) x (#NT x 325g/mol) x 10-6
  • from ug to pmol: pmol = (# of ug) x (#NT x 325g/mol) x 106

    A few Common Phosphoramidite Dilutions:
    Amidite MW Unit MW 0.05 M vol(ml) of diluent per 1gm bottle 0.05 M vol(ml) of diluent per 1gm bottle
    dA(bz)
    dC(bz)
    dG(ibu)
    T
    2'-d-Inosine
    dU
    2'-Si-A(bz)
    2'-Si-C(bz)
    2'-Si-G(ibu)
    2'-Si-U
    2'-Si-I
    2'-OMe-A
    2'-OMe-C
    2'-OMe-G
    2'-OMe-U
    		 857.95
    833.93
    839.94
    744.83
    754.79
    730.80
    988.18
    964.15
    970.16
    861.03
    885.06
    887.97
    863.95
    869.97
    760.81
    		 313.21
    289.19
    329.21
    304.20
    314.19
    290.17
    329.21
    305.19
    345.21
    306.17
    330.19
    343.24
    319.21
    359.24
    320.20
    		 23.3
    23.9
    23.8
    26.8
    26.5
    27.4
    20.2
    20.7
    20.6
    23.2
    22.6
    22.5
    23.1
    23.0
    26.3
    		 11.7
    12.0
    11.9
    13.4
    13.3
    13.7
    10.1
    10.4
    10.3
    11.6
    11.3
    11.3
    11.6
    11.5
    13.2


















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